MRS BROTH (DE MAN, ROGOSA, SHARPE) Oxoid Katalog CM0359B Kemasan 500 gram A non-selective medium for profuse growth of `lactic acid bacteria’. Directions Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes. MRS Broth may be used for tests in the identification of lactobacilli, such as temperature dependence, growth in 4% NaCl, growth in 0.4% Teepol, etc. as recommended by Sharpe, Fryer and Smith1.