I2481C-1GR IPTG 1 gr GOLDBIO

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria. IPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue/white colony screening or Magenta-Gal (Gold Bio #B-378) for red/white colony screening of bacterial colonies. IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags). GoldBio IPTG is made from a synthetic, non-animal origin. Product Specifications: IPTG Isopropyl-beta-D-thiogalactoside MOLECULAR BIOLOGY GRADE Forumla: C9H18O5S MW: 238.30 g/mol Purity: gt;99% by HPLC Dioxane free Storage/Handling: Store desiccated at -20°C. Protect from light. PubChem Chemical ID: 656894 Product Specifications Catalog ID I2481 Name(s) IPTG Isopropyl-beta-D-thiogalactoside CAS # 367-93-1 Formula C9H18O5S MW 238.30 g/mol Grade MOLECULAR BIOLOGY GRADE Storage/Handling Store desiccated at -20°C. Protect from light.