Rappaport Vassiliadis Medium 500gr

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Code: M880

Recommended for enrichment of Salmonellae, based on its ability to multiply selectively at high osmotic pressure, low pH and at 43°C, with modest nutritional requirements.

Directions
Suspend 49.17 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired into tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.

Principle And Interpretation
Rappaport Vassiliadis Medium is designed according to the revised formulation by Van Schothorst et al (5) and is recommended for the selective enrichment of Salmonellae from food and environmental specimens. Present medium is a modification of the Rappaport Vassiliadis Enrichment Broth described by Van Schothorst and Renauld (6). Addition of magnesium chloride to the medium was reported by Peterz et al (3). Salmonella species can be isolated from human faeces without pre-enrichment by using this medium.